Next, approximately half of the top plasma layer in the blood bottle is aspirated into a syringe, and the remainder of the top plasma layer, together with the middle white buffy coat layer, is aspirated into a second syringe.
The first syringe is then placed in a heated container, denaturing the protein (mostly albumin) into a thickened, gel-like consistency, whilst the second syringe is placed in a cooling container, maintaining its liquid consistency as per our protocol.
At the end of this stage, the contents of the two syringes are combined using a double female connector.
The combined mixture is then injected mainly into layers 2 and 5 of the skin. Injection into layer 5, over bone, provides immediate support for the layers above whilst, over time, rejuvenating the bone and deep fat that decrease with ageing. Injection of the mixture into layer 2, the dermis layer where the fibroblast cells reside, stimulates the fibroblasts to secrete new collagen, elastin and hyaluronic acid (HA), which plumps up the skin, making it appear youthful, whilst also stimulating the basal layer of the epidermal cells to give the skin a brighter glow.
Both PRP and PRF are broken down, or resorbed, over time. However, the resorption time for PRF, compared with PRP, is extended from two to three weeks to approximately four to six months.